Journal:

Article Title: Surface Display of Foreign Epitopes on the Lactobacillus brevis S-Layer

doi: 10.1128/AEM.68.12.5943-5951.2002

Figure Lengend Snippet: Strains and plasmids used in this study

Article Snippet: Four putative epitope insertion sites in the slpA gene of L. brevis ATCC 8287 (designated L. brevis GRL1 below) were chosen on the basis of the hydrophilicity profile of the SlpA protein analyzed by the PC-Gene program Antigen (Genofit, Geneva, Switzerland).

Techniques: Plasmid Preparation, Expressing, Clone Assay

Journal:

Article Title: Surface Display of Foreign Epitopes on the Lactobacillus brevis S-Layer

doi: 10.1128/AEM.68.12.5943-5951.2002

Figure Lengend Snippet: PCR analysis of the slpA-c-myc gene replacement in L. brevis GRL1046. (A) Total chromosomal DNA of the GRL1046 (lanes 2, 4, 6, 7, and 10) and GRL1 wild-type (lanes 1, 3, 5, and 9) strains and pORI280 plasmid DNA (lane 8) were used as templates in PCR with the primer pairs p14 and p21 (lanes 1 and 2), p22 and p4 (lanes 3 and 4), p22 and p23 (lanes 5 and 6), p24 and p25 (lanes 7 and 8) and p26 and p13 (lanes 9 and 10). (B) Total DNA of GRL1 (lane 1) and GRL1046 (lane 2) amplified with primers p27 and p28. PCR products were separated by electrophoresis on a 0.8% (A) or 2% (B) agarose gel and stained with ethidium bromide. Values of the molecular size markers (SmartLadder; Eurogentec) are indicated on the left (A) or on the right (B).

Article Snippet: Four putative epitope insertion sites in the slpA gene of L. brevis ATCC 8287 (designated L. brevis GRL1 below) were chosen on the basis of the hydrophilicity profile of the SlpA protein analyzed by the PC-Gene program Antigen (Genofit, Geneva, Switzerland).

Techniques: Plasmid Preparation, Amplification, Electrophoresis, Agarose Gel Electrophoresis, Staining

Journal:

Article Title: Surface Display of Foreign Epitopes on the Lactobacillus brevis S-Layer

doi: 10.1128/AEM.68.12.5943-5951.2002

Figure Lengend Snippet: Southern blot analysis of L. brevis strains GRL1046 and GRL1. Total DNA extracted from strains GRL1046 (lane1) and GRL1 (lane 2) was digested with EcoRI and hybridized with a 1,406-bp internal slpA fragment probe amplified with the primer pair p19 and p20. Values (in kilobase pairs) of the molecular size markers (SmartLadder) are indicated on the left.

Article Snippet: Four putative epitope insertion sites in the slpA gene of L. brevis ATCC 8287 (designated L. brevis GRL1 below) were chosen on the basis of the hydrophilicity profile of the SlpA protein analyzed by the PC-Gene program Antigen (Genofit, Geneva, Switzerland).

Techniques: Southern Blot, Amplification

Journal:

Article Title: Surface Display of Foreign Epitopes on the Lactobacillus brevis S-Layer

doi: 10.1128/AEM.68.12.5943-5951.2002

Figure Lengend Snippet: SDS-PAGE analysis of L. brevis GRL1 and GRL1046 cells. Equal amounts of GRL1 (lane 1) and GRL1046 (lane 2) cells from an OD600 of 0.8 were boiled in Laemmli sample buffer before being subjected to protein gel analysis. Values of the molecular mass marker proteins are indicated on the left.

Article Snippet: Four putative epitope insertion sites in the slpA gene of L. brevis ATCC 8287 (designated L. brevis GRL1 below) were chosen on the basis of the hydrophilicity profile of the SlpA protein analyzed by the PC-Gene program Antigen (Genofit, Geneva, Switzerland).

Techniques: SDS Page, Marker

Journal:

Article Title: Surface Display of Foreign Epitopes on the Lactobacillus brevis S-Layer

doi: 10.1128/AEM.68.12.5943-5951.2002

Figure Lengend Snippet: Whole-cell ELISA for detection of surface-exposed c-Myc epitope in L. brevis GRL1046. Anti-Myc antibody was allowed to bind to lactobacillar cells, after the addition of a horseradish peroxidase conjugate, different lactobacillar cell densities were incubated with a chromogenic substrate. Symbols: •, GRL1046; ▴, GRL1 wild-type strain.

Article Snippet: Four putative epitope insertion sites in the slpA gene of L. brevis ATCC 8287 (designated L. brevis GRL1 below) were chosen on the basis of the hydrophilicity profile of the SlpA protein analyzed by the PC-Gene program Antigen (Genofit, Geneva, Switzerland).

Techniques: Enzyme-linked Immunosorbent Assay, Incubation

Journal:

Article Title: Surface Display of Foreign Epitopes on the Lactobacillus brevis S-Layer

doi: 10.1128/AEM.68.12.5943-5951.2002

Figure Lengend Snippet: Immunofluorescence microscopy of L. brevis GRL1046 (A) and wild-type L. brevis GRL1 (B). Bacteria harvested from overnight cultures were treated with anti- Myc antibodies (diluted 1:10 in PBS) and FITC-conjugated secondary antibody. Both pictures were taken after 5-s exposures. Magnification, ×4,000.

Article Snippet: Four putative epitope insertion sites in the slpA gene of L. brevis ATCC 8287 (designated L. brevis GRL1 below) were chosen on the basis of the hydrophilicity profile of the SlpA protein analyzed by the PC-Gene program Antigen (Genofit, Geneva, Switzerland).

Techniques: Immunofluorescence, Microscopy

Journal:

Article Title: Surface Display of Foreign Epitopes on the Lactobacillus brevis S-Layer

doi: 10.1128/AEM.68.12.5943-5951.2002

Figure Lengend Snippet: Transmission electron microscopy of L. brevis GRL1046 and GRL1 S-layers. Negative staining was performed with cells of GRL1046 (A) and GRL1 (B) derived from aged colonies and with S-layer protein preparations from LiCl isolations of GRL1046 (C) and GRL1 (D). Bars, 0.1 μm.

Article Snippet: Four putative epitope insertion sites in the slpA gene of L. brevis ATCC 8287 (designated L. brevis GRL1 below) were chosen on the basis of the hydrophilicity profile of the SlpA protein analyzed by the PC-Gene program Antigen (Genofit, Geneva, Switzerland).

Techniques: Transmission Assay, Electron Microscopy, Negative Staining, Derivative Assay